Comparative transcriptomic and proteomic signature of lung alveolar macrophages reveals the integrin CD11b as a regulatory hub during pneumococcal pneumonia infection

Introduction Streptococcus pneumoniae is one of the main causes community-acquired infections in lung alveoli children and elderly. Alveolar macrophages (AM) patrol homeostasis under infectious conditions. However, molecular adaptations AM upon with are incompletely resolved. Methods We used a comparative transcriptomic proteomic approach to provide novel insights into cellular mechanism that changes signature during infections. Using tandem mass spectrometry murine cell-sorted AM, we revealed significant infection pneumoniae. Results showed strong neutrophil-associated signature, such as expression CD11b, MPO, neutrophil gelatinases, elastases, which was associated phagocytosis recruited neutrophils. Transcriptomic analysis indicated intrinsic CD11b by AM. Moreover, profiling identified central hub influenced recruitment, activation, migration. Discussion In conclusion, our study provides reveals profound alterations critical for effective antimicrobial immunity.